Weāre trying to make the G3 journal a home for all your fun bacterial genetics stories! Please hit us up if you have a relevant manuscript to submit!
Weāre trying to make the G3 journal a home for all your fun bacterial genetics stories! Please hit us up if you have a relevant manuscript to submit!
The annoying thing when (in retrospect) you probably would have designed the experiment different now than then, and the reviewer says this, and you acknowledge it, but thereās still valid results in the data and you have no money to do the thing againā¦and so it stays a preprint forever
I'm not smart enough to know if keeping Joe or ditching Joe is the right call when it comes to defeating Donald Trump, but I _know_ Kamala would go on Hot Ones, and I doubt either of the other guys would, so that gives her the edge, I think.
Does anyone have an npt2 promoter sequence that can be synthesized into a plasmid (and works for expression?)
Or, other strong promoter that works across Gram negative bacteria?
TIA
My 2Ā¢:
There are going to be plenty of situations where you can tell who the authors are, but also situations where you or others canāt.
If bias in the review process (and studies suggest there is) and double blinding partially works to counter bias and not introduce new biases then it helps
Double-blind peer-review is a great idea, but in practice it doesn't quite work. I just received a review request and based on the topic/system I know exactly who the PI is
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A better campaign would understand that the debate was a fiasco and their campaign is currently on life-support, and respond to it with the urgency that requires. It's not about one thing here or there to "fix it" and move on. It's that they're in real trouble, and not responding to it urgently
A better campaign would understand that the debate was a fiasco and their campaign is currently on life-support, and respond to it with the urgency that requires. It's not about one thing here or there to "fix it" and move on. It's that they're in real trouble, and not responding to it urgently
Hereās why Biden is toast: in this situation, any candidate in Bidenās shoes would do a series of events (interviews, press conferences, town halls, etc) to prove they are up to the task.
But, Biden isnāt doing that because he canāt. Heās not capableāand thatās not going to change.
Completely aware no one is listening but Iām finding it at least slightly cathartic to respond to every Biden fundraising text by saying he should drop outā¦
Youāre the Presidentā¦do ***something*** quickly to counter the narrative or get the hell out of the way
Press conference back and forthā¦something that shows youāre up for actually trying to win this thing
Youāre the Presidentā¦do ***something*** quickly to counter the narrative or get the hell out of the way
Press conference back and forthā¦something that shows youāre up for actually trying to win this thing
OK @plasmidsaurus.bsky.social ā¦
I mean I was blown away by plasmid sequencing by the next day, but I shipped bacterial genomic DNA at 4PM yesterday and got back whole genome sequences 10AM the next day
Pedantry corner:
Transposons writ large are not āinstruments of horizontal gene transferā
They can associate with regions of DNA that can transfer and so theyāre over represented amongst HGTād genes but a lot of them aināt transferring themselves
If Biden literally shat himself during one of the debates, his opponent would still be the guy openly vowing to send federal troops to take over cities, set up mass detention camps, and imprison his critics. The only sane position would still be Team Pants Shitter.
My marriage, in a nutshell:
Me: āHowād your day go honey?ā
J: āMind if I go to the Olympics in Paris in August? Friend has tickets and a roomā
Me: āIāll get the kids to school that week, go for it, canāt pass up once in a lifetime thingsā
Its promotion letter season!
Remember, your job is to give the promotion committee snippets that they can pull out and highlight for the packet while not saying anything that could be misconstrued poorly
Hi friends! I have a trans friend who is going to be traveling cross-country and they're kind of worried about getting stuck in places they won't be safe. Are there any up-to-date safe travels guides for trans people anywhere, like routes to take and towns to avoid at all costs? Thanks all š„°